Generator

Part:BBa_K1364008:Design

Designed by: Laureen Mirassou   Group: iGEM14_Toulouse   (2014-10-01)


Pveg - strong RBS - Antifungal GAFP-1 - Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of the strong, constitutive promoter of Bacillus subtilis Pveg (K823003), strong RBS (K780002), the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) and a double terminator B0015. optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.

BBa_K1364008.png

Source

This part results from the association of BBa_K143012, BBa_K1364002 and BBa_B0015.

References